LOCUS       40924_23499       12221 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB624 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12221)
  AUTHORS   Nakamura S, Mano S, Tanaka Y, Ohnishi M, Nakamori C, Araki M, Niwa 
            T, Nishimura M, Kaminaka H, Nakagawa T, Sato Y, Ishiguro S.
  TITLE     Gateway binary vectors with the bialaphos resistance gene, bar, as a
            selection marker for plant transformation
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (6), 1315-1319 (2010)
  PUBMED    20530878
REFERENCE   2  (bases 1 to 12221)
  AUTHORS   Tanaka Y, Nakamura S, Ishiguro S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12221)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12221)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2010"; volume: "74"; issue: "6"; 
            pages: "1315-1319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1831
                     /codon_start=1
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             1838..1855
                     /codon_start=1
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
                     /translation="LVPRGS"
     protein_bind    1865..1989
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2026..2056
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2110..2766
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3111..3413
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3457..3581)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3607..3859
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3893..3909)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3917..3933
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3941..3971)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3986..4007)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(4043..4295)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4458..5006)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(5058..5237)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5743..5767)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6288..7076
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7325..7913
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8099..8239)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8583..8777)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8846..9916)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10348..10974)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg tcccctatac taggttattg
     1201 gaaaattaag ggccttgtgc aacccactcg acttcttttg gaatatcttg aagaaaaata
     1261 tgaagagcat ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt
     1321 gggtttggag tttcccaatc ttccttatta tattgatggt gatgttaaat taacacagtc
     1381 tatggccatc atacgttata tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga
     1441 gcgtgcagag atttcaatgc ttgaaggagc ggttttggat attagatacg gtgtttcgag
     1501 aattgcatat agtaaagact ttgaaactct caaagttgat tttcttagca agctacctga
     1561 aatgctgaaa atgttcgaag atcgtttatg tcataaaaca tatttaaatg gtgatcatgt
     1621 aacccatcct gacttcatgt tgtatgacgc tcttgatgtt gttttataca tggacccaat
     1681 gtgcctggat gcgttcccaa aattagtttg ttttaaaaaa cgtattgaag ctatcccaca
     1741 aattgataag tacttgaaat ccagcaagta tatagcatgg cctttgcagg gctggcaagc
     1801 cacgtttggt ggtggcgacc atcctccaaa atcggatctg gttccgcgtg gatccgctgt
     1861 tatcacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
     1921 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     1981 gtcactatgg cggcccctcg aggggccgca ttaggcaccc caggctttac actttatgct
     2041 tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag
     2101 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     2161 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     2221 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     2281 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
     2341 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     2401 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
     2461 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     2521 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
     2581 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     2641 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
     2701 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
     2761 ggggcgtaaa cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc
     2821 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga
     2881 ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
     2941 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg
     3001 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
     3061 gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta
     3121 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
     3181 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
     3241 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
     3301 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
     3361 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
     3421 caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt
     3481 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
     3541 tcattttacg tttctcgttc agctttcttg tacaaagtgg tgataaccta gagctcgaat
     3601 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     3661 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     3721 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     3781 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     3841 tcatctatgt tactagatcg ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc
     3901 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     3961 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaggc tgaattaggc
     4021 gcgcctattt ctattaattc ccgatctagt aacatagatg acaccgcgcg cgataattta
     4081 tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact
     4141 ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc
     4201 ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat
     4261 cttaagaaac tttattgcca aatgtttgaa cgatcgggga aattcgagct ggggacgtcc
     4321 ccagctcggt accccctcct gggcacggtc gtacgcgatc cgccgcggtt ccacctcacc
     4381 attgcgggta tgccagttga gttcgatgag ttcccgtacc agctccaact cgggcgatgc
     4441 cacgcgcact ccgttcagat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg
     4501 ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc
     4561 cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg
     4621 ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg
     4681 tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc
     4741 gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg
     4801 gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg
     4861 tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg
     4921 atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg
     4981 tcggccgggc gtcgttctgg gctcatatct tattgccccc cgggatcaga tctggattga
     5041 gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat
     5101 ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata
     5161 atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc tgagtggctc
     5221 cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg
     5281 ggggtcataa cgtgactccc ttaattctcc gctcatgatc ttgatcccct gcgccatcag
     5341 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
     5401 ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat
     5461 cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc
     5521 cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc
     5581 tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgaagct
     5641 gatccgtcga gattaataga aataaattca gcctaattcg gcgttaattc agtacattaa
     5701 aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat
     5761 cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga
     5821 tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac
     5881 tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca
     5941 cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt
     6001 gatcaattcg ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     6061 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     6121 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     6181 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     6241 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg
     6301 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     6361 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     6421 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     6481 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     6541 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     6601 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     6661 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     6721 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     6781 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     6841 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     6901 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     6961 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     7021 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat
     7081 gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt
     7141 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta
     7201 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa
     7261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7441 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     7501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7981 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8041 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8101 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     8161 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     8221 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     8281 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     8341 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
     8401 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     8461 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     8521 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     8581 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     8641 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     8701 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     8761 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt
     8821 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     8881 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     8941 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     9001 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     9061 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     9121 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     9181 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     9241 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     9301 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     9361 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     9421 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     9481 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     9541 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     9601 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
     9661 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
     9721 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
     9781 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
     9841 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
     9901 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
     9961 gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
    10021 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
    10081 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
    10141 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
    10201 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
    10261 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
    10321 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
    10381 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
    10441 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
    10501 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
    10561 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
    10621 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
    10681 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
    10741 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
    10801 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
    10861 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
    10921 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
    10981 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
    11041 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
    11101 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
    11161 cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc
    11221 tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt
    11281 gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct
    11341 cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg
    11401 cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc
    11461 cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg
    11521 ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga
    11581 ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg
    11641 gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga
    11701 tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca
    11761 ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc
    11821 gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg
    11881 cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag
    11941 cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta
    12001 tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg
    12061 taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc
    12121 cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga ggggaaccct
    12181 gtggttggca tgcacataca aatggacgaa cggataaacc t
//
